Pujols was hit directly in the elbow while swinging at a pitch in the third inning, and had his arm on ice following the 4-1 loss. The pitch from Royals starter Will Smith looked like a fastball, but cut late and struck Pujols in the arm.
X-Rays taken on Pujols' arm on Wednesday were negative.
"It just caught him flush," Angels manager Mike Scioscia said. "It was really weird, the ball looked like it was a fastball in, and the last second it had a big horizontal cut to it. He started to swing and it tracked him into the elbow."
With Pujols out on Wednesday, Kendrys Morales started at first base and Torii Hunter, who bruised his ribs while diving for a ball in the sixth inning of Tuesday's game, served as the designated hitter. Scioscia said that Pujols would likely not play, even as a pinch-hitter.
"He was sore today, and we'll evaluate, and hopefully he'll come out of the day off [Thursday] and be ready to go on Friday [against the Rays]," Scioscia said.
During his last 55 games, Pujols is batting .331 with 15 home runs and 43 RBIs. He was batting .212 with three homers and 18 RBIs through his first 42 games this season. Pujols has started 97 of the team's 99 games this season.